Cytotoxic T Cells and Neutralizing Antibodies Induced in Rhesus Monkeys by Virus-like Particle HIV Vaccines in the Absence of Protection from SHIV Infection
Identifieur interne : 003B80 ( Main/Exploration ); précédent : 003B79; suivant : 003B81Cytotoxic T Cells and Neutralizing Antibodies Induced in Rhesus Monkeys by Virus-like Particle HIV Vaccines in the Absence of Protection from SHIV Infection
Auteurs : Ralf Wagner [Allemagne] ; Vera J. P. Teeuwsen [Pays-Bas] ; Ludwig Deml [Allemagne] ; Frank Notka [Allemagne] ; Anthonius G. M. Haaksma [Pays-Bas] ; Sunita S. Jhagjhoorsingh [Pays-Bas] ; Henk Niphuis [Pays-Bas] ; Hans Wolf [Allemagne] ; Jonathan L. Heeney [Pays-Bas]Source :
- Virology [ 0042-6822 ] ; 1998.
English descriptors
- Teeft :
- Adjuvant, Amino, Amino acids, Antibody titers, Autologous, Binding region, Booster, Booster immunization, Chimeric, Control animals, Ctls, Cytotoxic, Cytotoxicity, Deml, Different time points, Effector, Epitope, First booster immunization, Glycoprotein, Human immunodeficiency virus type, Humoral, Hybrid, Immune responses, Immunization, Immunodeficiency, Immunodeficiency virus type, Immunogenicity, Insect cells, Lymphocyte, Lymphocyte response, Lysed target cells, Lysis, Macaque, Neutralizing, Neutralizing antibodies, Outer surface, Pbmc, Pbmc cocultures, Peptide, Peptide pools, Precursor, Quantitative virus isolation, Recombinant, Rhesus, Rhesus macaques, Rhesus monkeys, Second booster immunization, Second immunization, Shiv, Simian immunodeficiency virus, Single peptides, Specific lysis, Stimulator cells, Time point, Titer, Unpulsed, Unpulsed targets, Vaccine, Virol, Virology, Virus load, Vlps.
Abstract
Abstract: HIV Pr55gaghas in the absence of other viral components the capacity to self assemble in budding noninfectious virus-like particles (VLP). The immunological spectrum of the HIV-1IIIBgag-derived VLP was expanded either by stable anchoring of chimeric modified gp120 on the surface of the VLP (type 1) or by replacing sequences of the Pr55gagprecursor by the V3 loop and a linear portion of the CD4 binding domain (type 2). This noninfectious antigen delivery system was evaluated for immunogenicity and efficacy in rhesus macaques without adjuvants. Intramuscular immunization with both types of VLP induced high titers ofgag-specific antibodies ranging from 1/8000 to 1/510,000 for type 1 VLP and from 1/4000 to 1/16,000 for type 2 VLP. Only animals immunized with type 1 VLP developed substantial endpoint titers ofenv-specific antibodies (1/2000–1/32,000) with a neutralizing capacity at serum dilutions of 1/32–1/128.Gag- andenv-specific cytotoxic T lymphocyte (CTL) activity was induced by both types of VLP at similar levels. Four weeks after the last immunization animals were challenged intravenously with 20 MID50of the cell free homologous envelope simian/HIV-1IIIBchimeric challenge stock. Despite HIV-1-specific neutralizing and CTL responses, all vaccinated animals became infected.
Url:
DOI: 10.1006/viro.1998.9104
Affiliations:
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M." last="Haaksma">Anthonius G. M. Haaksma</name><affiliation wicri:level="1"><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Department of Virology, Biomedical Primate Research Centre, Rijswijk</wicri:regionArea><wicri:noRegion>Rijswijk</wicri:noRegion></affiliation></author><author><name sortKey="Jhagjhoorsingh, Sunita S" sort="Jhagjhoorsingh, Sunita S" uniqKey="Jhagjhoorsingh S" first="Sunita S." last="Jhagjhoorsingh">Sunita S. Jhagjhoorsingh</name><affiliation wicri:level="1"><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Department of Virology, Biomedical Primate Research Centre, Rijswijk</wicri:regionArea><wicri:noRegion>Rijswijk</wicri:noRegion></affiliation></author><author><name sortKey="Niphuis, Henk" sort="Niphuis, Henk" uniqKey="Niphuis H" first="Henk" last="Niphuis">Henk Niphuis</name><affiliation wicri:level="1"><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Department of Virology, Biomedical Primate Research Centre, Rijswijk</wicri:regionArea><wicri:noRegion>Rijswijk</wicri:noRegion></affiliation></author><author><name sortKey="Wolf, Hans" sort="Wolf, Hans" uniqKey="Wolf H" first="Hans" last="Wolf">Hans Wolf</name><affiliation wicri:level="1"><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institut für Medizinische Mikrobiologie und Hygiene, Universität Regensburg, Regensburg</wicri:regionArea><wicri:noRegion>Regensburg</wicri:noRegion><wicri:noRegion>Regensburg</wicri:noRegion></affiliation></author><author><name sortKey="Heeney, Jonathan L" sort="Heeney, Jonathan L" uniqKey="Heeney J" first="Jonathan L." last="Heeney">Jonathan L. Heeney</name><affiliation wicri:level="1"><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Department of Virology, Biomedical Primate Research Centre, Rijswijk</wicri:regionArea><wicri:noRegion>Rijswijk</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j">Virology</title><title level="j" type="abbrev">YVIRO</title><idno type="ISSN">0042-6822</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1998">1998</date><biblScope unit="volume">245</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="65">65</biblScope><biblScope unit="page" to="74">74</biblScope></imprint><idno type="ISSN">0042-6822</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0042-6822</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Adjuvant</term><term>Amino</term><term>Amino acids</term><term>Antibody titers</term><term>Autologous</term><term>Binding region</term><term>Booster</term><term>Booster immunization</term><term>Chimeric</term><term>Control animals</term><term>Ctls</term><term>Cytotoxic</term><term>Cytotoxicity</term><term>Deml</term><term>Different time points</term><term>Effector</term><term>Epitope</term><term>First booster immunization</term><term>Glycoprotein</term><term>Human immunodeficiency virus type</term><term>Humoral</term><term>Hybrid</term><term>Immune responses</term><term>Immunization</term><term>Immunodeficiency</term><term>Immunodeficiency virus type</term><term>Immunogenicity</term><term>Insect cells</term><term>Lymphocyte</term><term>Lymphocyte response</term><term>Lysed target cells</term><term>Lysis</term><term>Macaque</term><term>Neutralizing</term><term>Neutralizing antibodies</term><term>Outer surface</term><term>Pbmc</term><term>Pbmc cocultures</term><term>Peptide</term><term>Peptide pools</term><term>Precursor</term><term>Quantitative virus isolation</term><term>Recombinant</term><term>Rhesus</term><term>Rhesus macaques</term><term>Rhesus monkeys</term><term>Second booster immunization</term><term>Second immunization</term><term>Shiv</term><term>Simian immunodeficiency virus</term><term>Single peptides</term><term>Specific lysis</term><term>Stimulator cells</term><term>Time point</term><term>Titer</term><term>Unpulsed</term><term>Unpulsed targets</term><term>Vaccine</term><term>Virol</term><term>Virology</term><term>Virus load</term><term>Vlps</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: HIV Pr55gaghas in the absence of other viral components the capacity to self assemble in budding noninfectious virus-like particles (VLP). The immunological spectrum of the HIV-1IIIBgag-derived VLP was expanded either by stable anchoring of chimeric modified gp120 on the surface of the VLP (type 1) or by replacing sequences of the Pr55gagprecursor by the V3 loop and a linear portion of the CD4 binding domain (type 2). This noninfectious antigen delivery system was evaluated for immunogenicity and efficacy in rhesus macaques without adjuvants. Intramuscular immunization with both types of VLP induced high titers ofgag-specific antibodies ranging from 1/8000 to 1/510,000 for type 1 VLP and from 1/4000 to 1/16,000 for type 2 VLP. Only animals immunized with type 1 VLP developed substantial endpoint titers ofenv-specific antibodies (1/2000–1/32,000) with a neutralizing capacity at serum dilutions of 1/32–1/128.Gag- andenv-specific cytotoxic T lymphocyte (CTL) activity was induced by both types of VLP at similar levels. Four weeks after the last immunization animals were challenged intravenously with 20 MID50of the cell free homologous envelope simian/HIV-1IIIBchimeric challenge stock. Despite HIV-1-specific neutralizing and CTL responses, all vaccinated animals became infected.</div></front></TEI><affiliations><list><country><li>Allemagne</li><li>Pays-Bas</li></country></list><tree><country name="Allemagne"><noRegion><name sortKey="Wagner, Ralf" sort="Wagner, Ralf" uniqKey="Wagner R" first="Ralf" last="Wagner">Ralf Wagner</name></noRegion><name sortKey="Deml, Ludwig" sort="Deml, Ludwig" uniqKey="Deml L" first="Ludwig" last="Deml">Ludwig Deml</name><name sortKey="Notka, Frank" sort="Notka, Frank" uniqKey="Notka F" first="Frank" last="Notka">Frank Notka</name><name sortKey="Wolf, Hans" sort="Wolf, Hans" uniqKey="Wolf H" first="Hans" last="Wolf">Hans Wolf</name></country><country name="Pays-Bas"><noRegion><name sortKey="Teeuwsen, Vera J P" sort="Teeuwsen, Vera J P" uniqKey="Teeuwsen V" first="Vera J. P." last="Teeuwsen">Vera J. P. Teeuwsen</name></noRegion><name sortKey="Haaksma, Anthonius G M" sort="Haaksma, Anthonius G M" uniqKey="Haaksma A" first="Anthonius G. M." last="Haaksma">Anthonius G. M. Haaksma</name><name sortKey="Heeney, Jonathan L" sort="Heeney, Jonathan L" uniqKey="Heeney J" first="Jonathan L." last="Heeney">Jonathan L. Heeney</name><name sortKey="Jhagjhoorsingh, Sunita S" sort="Jhagjhoorsingh, Sunita S" uniqKey="Jhagjhoorsingh S" first="Sunita S." last="Jhagjhoorsingh">Sunita S. Jhagjhoorsingh</name><name sortKey="Niphuis, Henk" sort="Niphuis, Henk" uniqKey="Niphuis H" first="Henk" last="Niphuis">Henk Niphuis</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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